8-Hydroxydeoxyguanosine (8-OHdG) levels in urinary samples of pesticide sprayers on exposure to organophosphorus pesticides

B. P. Mishra, Z. G. Badade, Bhupinder Kaur Anand, Jhansi Lakshmi Lingidi, Sapna Jaiswal


Background: Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules. DNA is probably the most biologically significant target of oxidative attack. Among numerous types of oxidative DNA damage the formation of 8-hydroxyguanosine (8-OHdG) is a sensitive biomarker of oxidative stress, an adduct formed as a result of biochemical reaction between ROS and DNA. Chronic exposure to Organophosphorus (OP) pesticides is implicated in many health conditions that result from the induction of oxidative stress, including cytogenetic damage. The main objective of the study was to evaluate the biochemical levels of 8-OHdG in spot urinary samples under the exposed OP pesticide sprayers and farm workers.

Methods: In this study, 51 male pesticide sprayers and 39 farm workers in the age group of 18-47 years having exposure  ranged from 3 to 15 years in duration were selected. The referents (n=31) were selected on the same criteria as well as they were never exposed to pesticides at any time. This study was conducted during the growing season (January, 2009 – September, 2010). The most commonly used OP pesticides like chlorpyriphos, Diazinon, Dimethioate, Monocrotofos etc., were used in this study. Urine samples from each participant were taken in sterile tubes and were stored at -200C till analysed. The concentration of 8-OHdG in samples were analyzed using ELISA.

Results: The urinary levels of 8-OHdG were found to be significantly higher in the farm workers and pesticide sprayers in contrast to the level observed in the control group (p<0.05). When the data was analyzed in the exposed groups in relation to duration of exposure it was found that both the farm workers and sprayers who were exposed to OP pesticides for less than 5 years showed the maximum mean values of 8-OHdG in comparison to those exposed to for more than 10 years.

Conclusions: In view of this regular bio monitoring studies in target human populations are imperative necessary due to frequent changes in pesticide formulations and introduction of newer pesticides. Despite that several life style factors may influence the urinary concentrations of 8-OHdG but still this non-invasive bio-marker 8-OHdG is preferred over other invasive techniques to evaluate the environmental and occupational exposure effect of OP pesticides on the genotoxicity of the exposed workers.


Organophosphorus pesticides (OPP), Reactive oxygen species (ROS), Malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8OHdG)

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Benz CC, Yau C. Ageing, oxidative stress and cancer: paradigms in parallax. Nat Rev Cancer. 2008;8:875-9.

Castillo-Cadena J, Tenorio-Vieyra LE, Quintana-Carbia AI et al. Determination of DNA damage in flouriculturists exposed to mixtures of pesticides. J Biomed Biotechnol. 2006;1-12.

Akhgari M, Abdollahi M, Kebryaeezadeh A, Hosseni R et al. Biochemical evidence for free radical- induced lipid peroxidation as a mechanism for subchronic toxicity of malathion in blood and liver of rats. Hum Exp Toxicol. 2003;22:205-11.

Jalali N, Pajoumand A, Abdollahi A, Shadina S, Pakravan N. Pesticide poisoning: one year report of Loghman-Hakim hospital Poison Center. Prog Med Res. 2003;1:1-9.

Lambert WE, Lasarev M, Muniz J, Scherer J et al. Variation in organophosphate pesticide metabolites in urine of children living in agricultural communities. Environ Health Perspect. 2005;113:504-8.

Lagorio S, Tagesson C, Forstiere F et al. Exposure to benzene and urinary concentrations of 8-OHdG, a biological marker of oxidative damage to DNA. Occup Environ Med. 1994;51:739-43.

Loft S, Poulsen HE. Cancer risk and oxidative DNA damage in man. J Mol Med. 1996;74:297-312.

Kasai H, Wamoto-Tanaka I, MiaMoto T et al. Lifestyle and urinary 8-OHdG, a marker of oxidative DNA damage; Effects of exercise, working conditions, meat intake, body mass index and smoking. Jpn J C Res. 2001;92:9-15.

Kissby GE, Muniz JF, Scherer J et al. Oxidative stress and DNA damage in agricultural workers. J Agro Med. 2009;14:206-14.

Lee CH, Kamijima M, Kim H et al. 8-hydroxydeoxy guanosine (8-OHdG) levels in human leukocyte and urine according to exposure to organo phosphoros pesticides and paraoxonaze 1 genotype. Int arch Occup Environ Health. 2007;80:217-27.

Olinski R, Gackowski D, Roza Lski R, et al. Oxidative DNA damage in cancer patients; a cause or a consequence of the disease development. Muta Res. 2003;531:177-90.

McCauley LA, Lasarev MR, Higgins G, Rothlein J et al. Work characteristics and pesticide exposures among migrant agricultural families: a community- based research approach. Environ Health Perspect. 2001;109:533-8.

McCauley LA, Lasarev MR, Muniz JF, et al. Analysis of pesticide exposure and DNA damage in farm workers. J Agromed. 2008;13:237-46.

Muniz JF, McCauley LA and Kisby GE. Oxidative stress and DNA damage are key mechanisms of pesticide induced neuronal death. Society for Neuroscience. 2009;755:712-5.

Rynard SM. Urine mutagenecity assays. In: Hulka DS, Wilcosky TC, Griffth JD, Biological Markers in Epidemiology, New-York; Oxford University Press, 1990; 56-77.

Banerjee BD, Seth V, Ahmad RS. Pesticide induced oxidative stress; perspectives and trends. Rev Environ Health. 2001;16:1-40.

Bhalli JA, Ali T, Asimr, et al. DNA damage in Pakistani agricultural workers exposed to mixtures of pesticides. Environ mol Mutagen. 2009;50:37-45.

Bolognesi C. Genotoxicity of pesticides; A review of human bio-monitoring studies. Mutat Res. 2003;543:251-72.

Tope AM, Panemangalore Mina. Assessment of oxidative stress due to exposure to pesticides in plasma and urine of traditional limited resource farm workers; Formation of the DNA – adduct 8-OHdG. 2007;42:151-5.